Fig. 7.

Knockdown of miR-330-3p activates the COPB2/PI3K/AKT axis and blocks the inhibitory function of sh-KDM5A. A transfection efficacy of miR-330-3p in DU145 and PC-3 cells examined by RT-qPCR; B protein level of COPB2 and the phosphorylation of PI3K and AKT in DU145 and PC-3 cells after sh-KDM5A and miR-330-3p inhibitor transfections examined by western blot analysis; C protein levels of KDM5A and COPB2 and phosphorylation of PI3K and AKT in DU145 and PC-3 cells after Recilisib or DMSO treatment determined by western blot analysis; D proliferation ability of DU145 and PC-3 cells examined by the CCK-8 assay; E colony formation of DU145 and PC-3 cells detected by the colony formation assay; F DNA replication ability of cells measured by the EdU labeling assay; G apoptosis rate of DU145 and PC-3 cells detected by flow cytometry; H migration ability of DU145 and PC-3cells measured by the scratch test; I invasiveness of DU145 and PC-3 evaluated by the Transwell assay; J angiogenesis ability of HUVECs in different CM detected by tube formation assay. Data were collected from three independent experiments and expressed as mean ± SEM. Differences were analyzed by two-way ANOVA (A–J); #p < 0.05 compared to sh-NC; *p < 0.05 compared to sh-KDM5A + NC inhibitor or sh-KDM5A + DMSO