Fig. 8.

Knockdown of KDM5A blocks the inhibition of ETS1 on miR-330-3p and suppresses the COPB2/AKT axis to limit the tumor growth in vivo. A weekly change of the xenograft tumors in nude mice; B weight of the xenograft tumors on week 4; C protein levels of ETS1, KI67, VEGFA and CCND1, and phosphorylation of AKT in the collected tumor tissues examined by IHC; D, E number of metastatic nodules in mouse lung and liver tissues examined by HE staining in the setting of caudal vein injection of DU145 cells. Data were collected from three independent experiments and expressed as the mean ± SEM. Differences were analyzed by one-way ANOVA (B, D and E) or two-way ANOVA (A and C); *p < 0.05 compared to sh-NC; #p < 0.05 compared to sh-KDM5A + NC inhibitor