Table 3.
Antibodies used for western blot analysis
| Antibodies | Dilution | Item number | Producer |
|---|---|---|---|
| Primary antibodies | |||
| Bax | 1:1000 | ab32503 | Abcam |
| Bcl-2 | 1:1000 | ab32124 | Abcam |
| E-cadherin | 1:1,0000 | ab40772 | Abcam |
| Snail | 1:1000 | ab216347 | Abcam |
| H3K4me2 | 1:500 | ab32356 | Abcam |
| GAPDH | 1:1,0000 | ab181603 | Abcam |
| COPB2 | 1:2000 | ab192924 | Abcam |
| ETS1 | 1:1000 | 14,069 | CST |
| PI3K | 1:1000 | 4257 | CST |
| p-PI3k (Y607) | 1:1000 | ab182651 | Abcam |
| AKT | 1:1000 | 4685 | CST |
| p-AKT (Ser473) | 1:2000 | 4060 | CST |
| KDM5A | 1:5000 | ab194286 | Abcam |
| KDM5B | 1:1000 | ab181089 | Abcam |
| KDM5C | 1:2000 | ab259913 | Abcam |
| KDM5D | 1:1000 | #78,995 | CST |
| Secondary antibodies | |||
| Goat anti-rabbit IgG | 1:2000 | ab205718 | Abcam |
| Goat anti-mouse IgG | 1:5000 | ab205719 | Abcam |
Bax Bcl-2-associated X, Bcl-2 B-cell lymphoma-2, GAPDH glyceraldehyde-3-phosphate dehydrogenase, COPB2 COPI coat complex subunit β 2, ETS1 ETS proto-oncogene 1, PI3K phosphatidyl inositol 3-kinase, AKT protein kinase B, IgG Immunoglobulin G, Abcam Abcam Inc., Cambridge, MA, USA, CST Cell Signaling Technology, Beverly, MA, USA