FIG. 3.

Antifungal activity of FeCl₃-loaded J774 (a) and THP1 (b) cells, activated by IFN-γ plus LPS. P. marneffei conidia at a ratio of 1:2 were phagocytosed by J774- or PMA-differentiated THP1 cells for 2 h. After removal of nonphagocytosed conidia, cultures were incubated for a further 48 h (37°C, 5% CO₂) and stimulated with IFN-γ plus LPS. Cells were then washed and lysed, and live fungi were plated in YMA for 48 h. The results are expressed as percent CFU (mean + standard deviation) compared to the controls at 2 h (a) and as mean CFU ± standard deviation (b). Supernatants of J774 cells were assayed for nitrite content with the Griess reagent. ∗, P < 0.05, compared to control medium (by analysis of variance).