TABLE 1.
Effects of immunological activation and of NO on the survival of P. marneffei conidia phagocytosed by human or mouse macrophages
| Treatment | Effect of treatment on antifungal activity of:
|
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|---|---|---|---|---|---|---|
| Human THP1 cells
|
Mouse J774 cells
|
|||||
| CFU (102)a | % Yeasts | Nitrite (μM)b | CFU (102)a | % Yeasts | Nitrite (μM)b | |
| None (medium only) | 96.7 ± 12 | 75.6 | 0 | 207 ± 11 | 57.0 | 0 |
| SNP (200 μM) | 45.1 ± 6c | 60.4 | 8.9 ± 0.3 | 88 ± 10d | 27.2 | 12.3 ± 0.5 |
| IFN-γ + LPS (1 μg/ml)e | 53.6 ± 15c | 60.0 | 0 | 74 ± 9d | 36.5 | 19.6 ± 0.5 |
| IFN-γ + LPS + l-NMMA | ND | ND | ND | 179 ± 13 | 42.4 | 0 |
a
CFU in YMA after a 48-h culture. The results are expressed as the mean ± standard deviation of triplicate experiments.
b
Nitrite levels by the Griess reagent in 48-h supernatants.
c
P < 0.05, compared to control medium (by analysis of variance).
d
P < 0.01, compared to control medium (by analysis of variance).
e
The doses of IFN-γ were 50 and 500 U/ml for J774 and THP1 cells, respectively.