. Author manuscript; available in PMC: 2022 Dec 9.

Published in final edited form as: J Investig Med. 2004 Jan;52(1):62–71. doi: 10.1136/jim-52-01-29

TABLE 2.

Relationship between PKC activity and NO-Induced Apoptosis in MRL/lpr Spleen Lymphocytes

Deta NONOate (μM)	Apoptosis (% of Untreated Controls)		PKC Activity (% of Untreated Controls)
Deta NONOate (μM)	No PMA	PMA (10 ng/mL)	No PMA
0	100 ± 4	74 ± 4	100 ± 0
100	154 ± 13	109 ± 5	—
250	191 ± 18	132 ± 20	76 ± 27
500	253 ± 8	255 ± 6	—
1,000	233 ± 34	179 ± 4	39 ± 18

NO = nitric oxide; PKC = protein kinase C; PMA = phorbol myristate acid.

MRL/lpr spleen lymphocytes were cultured overnight in varying concentrations of Deta NONOate (0–1,000 μM) with or without PMA (10 ng/mL). Cells were analyzed by flow cytometry for the percentage of apoptotic cells present. The results reported are a percentage (± standard error) of the control wells (no additive) and were the average of triplicate readings from three different experiments. Cells were also analyzed for PKC activity in two separate experiments.