Table 1. Used EGFP positions and gRNA sequences of this study.

Subunit	EGFP position between AA	gRNA sequence
nAChRalpha1/CG5610	438D and 439L	ACAGATCGTCGTCGGCGCCC[GGG]
nAChRalpha2/CG6844	456G and 457L	CAGATTCAGCGGCTTGGTGG[GGG]
nAChRalpha4/CG12414	426M and 427D	AATAGCCGCCGTCCCCGATA[TGG]
AChRalpha5/CG32975	717G and 718S	CAGCACCCGAATGCCGGATG[CGG]
nAChRalpha6/CG4128	403T and 404A	TTACGCCGACGAGCCAATGG[CGG]
nAChRalpha7/CG32538	464G and 465S	GCAAGGGGATGACGGCAGCG[TGG]