Figure 1.

Generation of engineered OVs encoding trimerized OX40L and/or IL12

(A) Diagram showing the transformation of tumor cells into aAPCs to activate tumor-specific T cells through OV-mediated expression of OX40L and IL12 on tumor cells. (B) Schematic representation of HSV-1-based OVs encoding OX40L, IL12, or both proteins. The expression of functional OX40L by the infected SCC-15 tumor cells was analyzed (C) by using flow cytometry and (D) by monitoring the activation of cocultured OX40 reporter cells that expressed GFP upon OX40 signaling (n = 3). (E) Secretion of IL12 from the infected cells was detected by using an ELISA (n = 3). (F) The expression of functional IL12 by the infected SCC-15 tumor cells was analyzed by monitoring the activation of cocultured IL12 luciferase reporter cells (n = 3). The statistical analysis was determined by one-way ANOVA, followed by Tukey’s multiple comparison test analysis. All values are presented as the mean ± SEM. ∗∗∗∗p < 0.0001.