Figure 7.

Effect of OV-mOX40L/IL12-infected tumor cells on activating naive T cells

OT-I naive T cells (A, B) or OT-II naive T cells (C, D) were cocultured with MC38-OVA cells pretreated with OV-Δ34.5 or OV-mOX40L/IL12. T cells were stained and subjected to a flow cytometry analysis (A, C) and IFN-γ ELISpot assay (B, D) to profile T cell phenotype and activation status (n = 3). Statistical significance was determined by using one-way ANOVA, followed by Tukey’s multiple comparison test analysis. All values are presented as the mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.