Figure 1.

METTL3 was induced by I/R injury in BUMPT cells and C57/BL6 mice

BUMPT cells were treated with I/R (2/0, 2, and 4 h). C57/BL6 mice were subjected to bilateral renal ischemia for 28 min and reperfusion for 24 and 48 h. (A–C) qRT-PCR analysis of METTL3 expression in BUMPT cells, and the cortex and medulla of kidney. (D–F) The immunoblot analysis of METTL3 expression in BUMPT cells, and the cortex and medulla of kidney. (G–I) Analysis of the gray scale image between METTL3 and β-tubulin. (J) Immunofluorescence staining of METTL3 in BUMPT cells at indicated time points. Scale bar: 20 μM. (K) Quantification of METTL3 staining. (L and M) Immunohistochemical staining of METTL3 in kidney treated with or without I/R condition. Original magnification ×400. Scale bar: 100 μM. Data are expressed as means ± SD. (n = 6). ∗p < 0.05 versus sham or saline group.