Figure 4.

mmu_lncRNA 121686 mediated I/R-induced BUMPT cell apoptosis while hsa_lncRNA 520657 mediated I/R-induced HK-2 cells apoptosis

BUMPT and HK-2 cells were transfected with hsa_lncRNA 520657 sgRNA or mmu_lncRNA 121686 sgRNA and then were subjected to I/R (2/2 h). (A) Schema of Cas9 KO mmu_lncRNA 121686 and has_lncRNA 520657 (B and C) Flow cytometry analysis of BUMPT and HK-2 cells apoptosis. (D) Representative apoptosis rate of BUMPT cells and HK-2 cells. (E) Immunoblots analysis of the expression of cleaved caspase-3, caspase-3, and β-tubulin in BUMPT cells. (F) Grayscale analysis of western blot bands of cleaved caspase-3, caspase-3, and β-tubulin in BUMPT cells. (G) Immunoblots analysis of the expression of cleaved caspase-3, caspase-3, and β-tubulin in HK-2 cells. (H) Grayscale analysis of western blot bands of cleaved caspase-3, caspase-3, and β-tubulin in HK-2 cells. Data are expressed as means ± SD (n = 6). ∗p < 0.05 versus scramble with saline group. #p < 0.05 versus scramble with I/R group.