KO of mmu_lncRNA 121686 and hsa_lncRNA 520657 attenuated I/R-induced apoptosis, and this effect was reversed by miR-328-5p inhibitor
The KO cell lines of mmu_lncRNA 121686 and hsa_lncRNA 520657 were transfected with miR-328-5p inhibitor and then subjected to I/R treatment. (A) qRT-PCR analysis of the expression of mmu-miR-328-5p. (B) Flow cytometry analysis of BUMPT apoptosis. (C) The apoptosis rate of BUMPT cells. (D) Immunoblots analysis of the expression of cleaved caspase-3, caspase-3, HtrA3, and β-tubulin in BUMPT cells. (E) Grayscale analysis of western blot bands of cleaved caspase-3, caspase-3, HtrA3, and β-tubulin in BUMPT cells. (F) qRT-PCR analysis of the expression of hsa-miR-328-5p. (G) Flow cytometry analysis of HK-2 apoptosis. (H) The apoptosis rate of HK-2 cells. (I) Immunoblots analysis of the expression of cleaved caspase-3, caspase-3, HtrA3, and β-tubulin in HK-2 cells. (J) Grayscale analysis of western blot bands of cleaved caspase-3, caspase-3, HtrA3, and β-tubulin in HK-2 cells. Data are expressed as means ± SD (n = 6). ∗p < 0.05 versus scramble with saline group. #p < 0.05 versus I/R with scramble group. ▵p < 0.05 versus IR group with mmu_lncRNA 121686 or hsa_lncRNA 520657 KO group.