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. 2022 Aug 2;27(11):4575–4589. doi: 10.1038/s41380-022-01711-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A The double immunofluorescence staining showed the colocalization of PSD95 and HO-1, PSD95 and Syn in control, H₂O₂, H₂O₂ + miR-370-3p mimics, and H₂O₂ + anti-miR-370-3p group. B The expression of BACH1, HO-1, PSD95 and Syn proteins were analyzed in the HT22 cells transduced with miR-NC or miR-370-3p mimics for 48 h and treated with H₂O₂ (600 µmol/L) for 6 h by Western blot, n = 3 replication. BACH1: H₂O₂ vs. control, ****p < 0.0001, H₂O₂ + miR-370-3p mimics vs. H₂O₂ + miR-NC, ****p < 0.0001,;HO-1: H₂O₂ vs. control, ****p < 0.0001, H₂O₂ + miR-370-3p mimics vs. H₂O₂ + miR-NC, ***p < 0.001,;PSD95: H₂O₂ vs. control, ***p < 0.001, H₂O₂ + miR-370-3p mimics vs. H₂O₂ + miR-NC, *p < 0.05; Syn: H₂O₂ vs. control, ***p < 0.001, H₂O₂ + miR-370-3p mimics vs. H₂O₂ + miR-NC, *p < 0.05. One-way ANOVA followed by Tukey’s multiple comparisons test. C The expression of BACH1, HO-1, PSD95 and Syn were analyzed in the HT22 cells transduced with anti-miR-NC or anti-miR-370-3p for 48 h and treated with H₂O₂ (600 µmol/L) for 6 h by Western blot, n = 3 replications.BACH1: H₂O₂ vs. control, *p < 0.05, H₂O₂ + anti-miR-370-3p vs. H₂O₂ + anti-miR-NC, ***p < 0.001;HO-1; H₂O₂ vs. control, ****p < 0.0001,H₂O₂ + anti-miR-370-3p vs. H₂O₂ + anti-miR-NC, **p < 0.01; PSD95: H₂O₂ vs. control, ****p < 0.0001, H₂O₂ + anti-miR-370-3p vs. H₂O₂ + anti-miR-NC, **p < 0.01; Syn: H₂O₂ vs. control, ***p < 0.001, H₂O₂ + anti-miR-370-3p vs. H₂O₂ + anti-miR-NC, ***p < 0.001. One-way ANOVA followed by Tukey’s multiple comparisons test. D The content of ATP with miR-370-3p overexpression or knockdown in HT22 cells treated with H₂O₂ (600 µmol/L) for 6 h, n = 3 replications.H₂O₂ vs. control, ****p < 0.0001, H₂O₂ + miR-370-3p mimics vs. H₂O₂ + miR-NC, ***p < 0.001, H₂O₂ + anti-miR-370-3p vs. H₂O₂ + anti-miR-NC, **p < 0.01. One-way ANOVA followed by Tukey’s multiple comparisons test. E Representative fluorescent staining of Mitosox for mitochondrial ROS with miR-370-3p overexpression or knockdown in HT22 cells treated with H₂O₂ (600 µmol/L) for 6 h, and quantitative analysis of the mean optical density analysis of Mitosox, n = 3 replications. H₂O₂ vs. control,***p < 0.001,H₂O₂ + miR-370-3p mimics vs. H₂O₂, *p < 0.05,H₂O₂ + anti-miR-370-3p vs. H₂O₂, *p < 0.05, one-way ANOVA followed by Tukey’s multiple comparisons test. All data were represented as mean ± SEM.