Figure 3.

BDD expression in hepatocytes in vivo causes accumulation of amyloid-like aggregates that colocalize with FVIII in liver sections

Liver sections were prepared from mice injected with the indicated vector DNAs as described⁹ and stained for FVIII (red) and Thio-S (green).³² Shown are representative images from these analyses. Details of the vector constructs are depicted with plasmid maps on the left-hand side. The parental vector pMT³⁷ (Addgene) contains a transcription unit that is composed of the SV40 origin and enhancer element from the early promoter, the adenovirus major late promoter containing most of the tripartite leader present in adenovirus late mRNAs, a hybrid intron (IVS), the cytosolic dihydrofolate reductase (DHFR)-coding region, and the SV40 early polyadenylation signal (SV40 PolyA). The parental vector efficiently expresses DHFR.³⁷ Human BDD or N6 coding sequences were inserted upstream of the DHFR-coding region at the EcoR1 restriction sites (R1).