Figure 5.

BDD expression induces the UPR in mouse-passaged primary human hepatocytes (mpPHH)

mpPHH were transduced with a lentiviral vector expressing a codon-optimized form of SQ-BDD (Lenti-BDD) at the indicated vector doses and cultured in a hepatocyte-defined medium. The transduced mpPHHs were harvested for western blot analysis 10 days post-transduction. Each lane represents an individual culture well. The numerical numbers represent averaged fold changes after correction with loading control β-actin. Experiment was repeated twice with different batches of mpPHH and similar results were obtained.