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. 2022 Dec 9;12:21283. doi: 10.1038/s41598-022-24023-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MKL-1 induces the expression of PINK1-AS and ALDOA as a transcription factor. (a) The box diagram shows the level of MKL-1 mRNA in the Ualcan database (http://ualcan.path.uab.edu/analysis.htm). (b) Kaplan–Meier survival curves showing the effect of MKL-1 on overall survival. (c–h) WB and RT-qPCR to verify the efficiency of siRNA knockdown of MKL-1. Statistical data are shown. (i,g,k) WB and RT-qPCR to determine the expression level of ALDOA in Huh-7 and HepG2 cells after knockdown of MKL-1. Statistical data are shown. (l) RT-qPCR determination of PINK1-AS expression levels in Huh-7 and HepG2 cells after knockdown of MKL-1. (m,n) Correlation analysis showed that MKL-1 was positively correlated with the expression of ALDOA and PINK1-AS (P < 0.001). (o,p) Quantitative ChIP analysis to show direct binding of MKL-1 to the endogenous PINK1-AS and ALDOA promoter regions. (q,r) Prediction of MKL-1 binding sites in the human PINK1-AS and ALDOA promoters by gene sequence analysis. (s,t) Luciferase reporter gene test to detect whether MKL-1 can target the PINK1-AS and ALDOA promoters. *P < 0.05; **P < 0.01; ***P < 0.001.