FIG. 4.

Effect of adenylate cyclase toxin on phagocytosis. Phagocytosis studies were performed as described in the legend for Fig. 3, with nonopsonized (open bars) and opsonized (solid bars) bacteria. One hundred consecutive neutrophils were examined for the number of intracellular (green) bacteria. WT, wild-type GFP-labeled BP338; Cyc, GFP-labeled BPM3183; Both, unlabeled BP338 plus GFP-labeled BPM3183. Data were analyzed by the Student t test. Each bar represents the mean (± the standard error of the mean) from three independent experiments. #, significantly different from the Cyc strain (P < 0.05); ∗, significantly different from WT (P < 0.05).