TABLE 1.
Bordetella pertussis strains used in this study
| Strain | Phenotypic designation, description | Reference(s) |
|---|---|---|
| BP338 | Wild type, nalidixic acid-resistant Tohama (29), Fim2−a | 40 |
| BP347 | Bvg, avirulent BP338, Fim2− | 40 |
| BP353 | FhaA, BP338 with transposon insertion in FHA operon, Fim2− | 40 |
| BPM409 | FhaB, BP338 with transposon insertion in the FHA structural gene, Fim2− | 42 |
| BPM3183 | Cyc, BP338 with transposon insertion in adenylate cyclase toxin, Fim2− | 42 |
| BPM2041 | BrkA, BP338 with transposon insertion in BrkA, Fim2+ | 11, 42 |
| BPRA | Ptx, BP338 pertussis toxin deletion, streptomycin resistant, Fim2− | 3 |
| BPM1809 | Dnt, BP338 with transposon insertion in dermonecrotic gene, Fim2− | 42 |
| MLT7 | Lps, BP338 with transposon insertion in LPS operon, Fim2− | 36 |
| BP536 | Wild type, nalidixic acid-resistant, streptomycin-resistant Tohama, Fim2− | 2 |
| bplD | LpsD, BP536 LPS mutant, Fim2+ | 2 |
| bplG | LpsG, BP536 LPS mutant, Fim2+ | 2 |
| bplH | LpsH, BP536 LPS mutant, Fim2+ | 2 |
| bplL | LpsL, BP536 LPS mutant, Fim2+ | 2 |
| BBC8 | Wild type, streptomycin-resistant W28 (29), Fim2+ | 26 |
| BBC9 | Prn, BBC8 pertactin mutant, Fim2+ | 26 |
a
Fimbrial expression was evaluated using monoclonal antibodies BPD5 (against fimbria 2 [fim2]) and BPC10 (against fimbriae 3 and 6). None of the strains agglutinated with the sera against fimbriae 3 and 6.