Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 9;13:7624. doi: 10.1038/s41467-022-33576-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a E. coli lacZ is introduced into pXD69m2 vector as a reporter gene in E. lenta. b LacZ assays revealed a high dynamic range for the lacZ reporter system in E. lenta. β-Gal activity was normalized to Miller units. c Design of cumate-inducible expression systems using the CymR repressor and CuO, expressed under the control of Bacillus and E. lenta promoters in plasmids pXD70CT5 and pXD70CT3, respectively. d LacZ assays revealed tight regulation of cumate-inducible expression systems pXD70CT3 and pXD70CT5. e Design of IPTG-inducible expression system in plasmid pXD70LacZ6. f LacZ assays for pXD70LacZ6. Data in panels b, d, and f are represented as mean ± SD with n = 3 biological replicates. Source data are provided as a Source Data file.