Table 7.

Primers’ sequences and PCR conditions.

Gene	SNP	Primers (5′–3′)	PCR conditions
TGFB1	C-509T	F:GTCGCAGGGTGTTGAGTGACAG R:AGGGGGCAACAGGACACCTTA	95 °C, 5 min- initial denaturation, 95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles, 72 °C, 10 min- final elongation
TGFB1	Leu10Pro	F:CTCCGGGCTGCGGCTGCAGC R:GGCCTCGATGCGCTTCCGCTTCA	95 °C, 5 min- initial denaturation, 95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles, 72 °C, 10 min- final elongation
XRCC1	Arg280His	F:TGGGGCCTGGATTGCTGGGTCTG R:CAGCACCACTACCACACCCTGAAGG	94 °C, 5 min- initial denaturation, 94 °C, 40 s; 55 °C to 69 °C, 40 s; 72 °C, 30 s- 35 cycles, 72 °C, 10 min- final elongation
XRCC3	Thr241Met	F: GGTCGAGTGACAGTCCAAAC R: TGCAACGGCTGAGGGTCTT	95 °C, 5 min- initial denaturation, 95 °C, 1 min; 60 °C, 1 min; 72 °C, 1 min- 40 cycles, 72 °C, 5 min- final elongation