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. 2022 Dec 9;12:21354. doi: 10.1038/s41598-022-25461-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Landing and feeding on a host mimic in different visual contexts. (A) Schematic of the experimental apparatus. Groups of mosquitoes are enclosed in a glass cylinder positioned in front of an LCD monitor. To acclimate to the experimental conditions, mosquitoes are left unperturbed in front of a green background for 1 h. Control groups (top) were exposed to a second hour of green background during which the number of landing and feedings on a membrane feeder were quantified. Treatment groups (bottom) were exposed to randomly positioned squares following the patterns described in (B,C). (B) Proportion of feedings quantified at the end of the second hour of the experiment for the control (green, n = 8) and treatment groups (dark grey, crossed squares for square fields, and filled squares for regressing and expanding squares, 7 < n < 8). (C) Number of landings per mosquito quantified every five minutes of the second hour of the experiment for the control (green) and treatment groups (dark grey, crossed squares for square fields, and filled squares for regressing and expanding squares). Asterisks denote significant differences (***p < 0.001; generalized linear model).