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. 2022 Dec 10;12:508. doi: 10.1038/s41398-022-02274-5

Fig. 2. Altered proteostasis in human iPSC-neurons expressing the MAPT p.R406W mutation.

Fig. 2

A iPSC from a MAPT mutation carrier and CRISPR/Cas9-corrected control (wild-type (WT)) were differentiated into cortical neurons and cultured for 6 weeks prior to analysis. B Representative immunostaining for Tuj1 (green) and DAPI (blue) illustrates that at 6 weeks in culture, cells are enriched for neurons. Scale bar, 10 microns. C Immunoblots of cell lysates (10 μg total protein) were probed with LAMP1, Cathepsin D and ubiquitin antibodies. D Quantification of protein analyte levels in the MAPT p.R406W neurons and isogenic controls. Graph represents mean ± SEM. Significance was determined using an unpaired, t-test. *p < 0.05; **p < 0.01. ****p < 0.0001.