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. 2022 Dec 10;12:508. doi: 10.1038/s41398-022-02274-5

Fig. 4. Human iPSC-neurons expressing the MAPT p.R406W mutation display defects in vesicle acidity.

Fig. 4

Human iPSC-neurons neurons from a MAPT p.R406W mutation carrier and isogenic, CRISPR/Cas9-corrected control (wild-type (WT)) were differentiated into cortical neurons and cultured for 6 weeks prior to analysis. A MAPT p.R406W neurons exhibit reduced LysoTracker staining. Live cells were incubated with Lysotracker and were imaged as described in Methods. Representative images of LysoTracker-stained neurons are shown in gray scale for clarity. Scale bar, 10 microns. Lower panel represents magnification of the cells in the black box. B Schematic of the quantification of LysoTracker staining in MAPT p.R406W neurons (n = 78 cells) and isogenic controls (n = 46 cells). C Quantification of the intensity of LysoTracker staining in individual soma. Graphs represent mean ± SEM. Significance was determined using an unpaired, t-test. ***p < 0.001.