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. 2022 Dec 10;12:508. doi: 10.1038/s41398-022-02274-5

Fig. 6. Colocalization of tau and phospho-tau with LAMP1-positive vesicles.

Fig. 6

iPSC from a MAPT p.R406W mutation carrier and CRISPR/Cas9-corrected control (wild-type (WT)) were differentiated into cortical neurons and cultured for 6 weeks prior to analysis. A Representative confocal images showing colocalization of LAMP1 (red) and total tau (Tau5; green) or ptau (AT180; green) in the MAPT p.R406W neurons compared with isogenic controls. Scale bar, 10 microns. B Colocalization of LAMP1 and total tau in tau-positive soma (WT, n = 146 cells; p.R406W, n = 95 cells) C Colocalization of LAMP1 and ptau in ptau-positive soma (WT, n = 73 cells; p.R406W, n = 39 cells). Graphs represent mean ± SEM. Significance was determined using an unpaired, t-test. **p < 0.01; ****p < 0.0001.