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. 2022 Dec 10;12:508. doi: 10.1038/s41398-022-02274-5

Fig. 7. MAPT p.R406W organoids phenocopy lysosomal defects.

Fig. 7

A Schematic of organoid generation. iPSC from a MAPT mutation carrier and CRISPR/Cas9-corrected control (wild-type (WT)) were differentiated into cortical organoids and cultured for 2 months. B Enzyme activity of β-Glucuronidase measured in organoid lysates (10 μg protein) from MAPT p.R406W and corrected control organoids. Enzymatic activity was normalized to total protein. Graphs represent mean ± SEM. C Immunoblots of cell lysates from organoids were probed with LAMP1 and Cathepsin D (active form shown) antibodies. D Quantification of protein analyte levels in the MAPT p.R406W organoids and isogenic controls. Graphs represent mean ± SD. Significance was determined using an unpaired, t-test. *p < 0.05.