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. 2022 Dec 9;12:21313. doi: 10.1038/s41598-022-25176-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nlrp3^-/- mice show less inflammatory cell recruitment and reduced levels of IL-1β in the peritoneal lavage fluid in the thioglycollate-induced peritonitis model. Mice were injected intraperitoneally (i.p.) with 1 mL sterile 3% thioglycollate broth (closed circles) or 1 mL sterile PBS (open circles). Four hours afterward, intraperitoneal cells were collected, counted, and analyzed for neutrophils and macrophages by flow cytometry. In addition, peritoneal lavage fluid was collected for cytokine measurements. (A) Neutrophils and macrophages in the intraperitoneal lavage fluid were defined as Ly6G-positive cells and F4/80-positive cells, respectively. (B) The total cell number in the peritoneal lavage fluid of Nlrp3^+/+ and Nlrp3^−/− (PBS control, n = 3:3; Thioglycollate, n = 10:10). (C) The absolute numbers of neutrophils and macrophages in the peritoneal lavage fluid analyzed by flow cytometry (PBS control, n = 4:5; Thioglycollate, n = 10:10). (D) IL-1β in peritoneal lavage fluid measured by ELISA (PBS control, n = 3:3; Thioglycollate, n = 10:10).