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. 2022 Dec 9;12:21313. doi: 10.1038/s41598-022-25176-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NLRP3 regulates velocity of leukocytes rolling on the inflamed vessel walls. Intravital microscopy of mesenteric venules. (A) representative intravital microscopy images from a Nlrp3^+/+ (top) and Nlrp3^−/− (bottom) mouse treated with thioglycollate i.p. injection. All rolling leukocytes crossing the black vertical line through the mesenteric venule were counted. The white arrowheads points to the same leukocyte at 5 s interval. Supplementary videos are provided. (B) The number of rolling leukocytes on the vessel wall and the leukocyte velocity with or without thioglycollate treatment (PBS control, open circles, n = 3:3; Thioglycollate, closed circles, n = 11:8).