Table 3.
Antigenicity, allergenicity, solubility, and physicochemical property assessments of the primary sequence of multi-epitope-based vaccine construct
| Sl. No | Features | Assessment |
|---|---|---|
| 1 | Antigenicity | 0.5914 (probable ANTIGEN) |
| 2 | Allergenicity |
Probable non-allergen (AllerTOP v.2.0) Probable non-allergen (AllergenFP v.1.0) |
| 3 | Solubility | 0.717 (Soluble) |
| 4 | Number of amino acids | 231 |
| 5 | Molecular weight | 23055.04 Dalton |
| 6 | Theoretical isoelectric point (pI) | 7.83 |
| 7 | Total number of atoms | 3251 |
| 8 | Formula | C1012H1631N277O326S5 |
| 9 | Estimated half-life |
1 h (mammalian reticulocytes, in vitro) > 30 min (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
| 10 | Instability index | 26.87 (Stable) |
| 11 | Aliphatic index | 66.80 |
| 12 | Grand average of hydropathicity (GRAVY) | − 0.140 |