Table 1.

Examples of questions for possible data queries to guide the collection of experimental variables.

How do different transfection protocols and handling of cells effect numbers and accuracy of edited clones?

What is the effect of different guide RNA sequences on numbers and accuracy of edited clones

Are rapidly dividing clones more often associated with chromosomal abnormalities?

Is passage number or feeding schedule associated with chromosomal abnormalities?

At what passage number do chromosome abnormalities tend to arise?

Are off-target or additional sequences at the insertion site associated with phenotypic abnormalities?

Can we perform the same protocol and receive the same efficiency and accuracy of editing?

Do different cell types show consistent differences in efficiency and accuracy of editing?