Skip to main content
. 2022 Dec 9;13:7613. doi: 10.1038/s41467-022-35390-3

Fig. 5. Single-cell transcriptomics shows rejuvenation of aged muscle tissue by rMetrnl treatment 4 days post-injury.

Fig. 5

a Graphical representation of the experimental design. b Combined UMAP reduction plotted in two dimensions with cell cluster identities labeled. c, d Cell-to-cell communication analysis assessing ligand/receptor pairings across cell types. Immune cells were chosen for ligand source and strength of interaction and the number of ligand/receptor pairs is indicated by chord thickness. Number of cells in a group is indicated by circle diameter. e Heatmap showing results from c, d. f Two-tailed t test was performed and FDR adjusted to infer cell-to-cell communication pathways upregulated with immune cell-expressed ligands and FAP-expressed receptors. g Subset analysis of FAP cells from b shown by UMAP reduction and separated by sample group (h). Dashed circle highlights distinct FAP population identified as overrepresented in old PBS cells. i Gene Set Enrichment Analysis was used to determine p value using GO (biological process) database on FAP cell subset circled from g, h when differential expression analysis was performed against all other FAP cells. Graphics created with BioRender.com. Source data available through GEO at Accession ID GSE217258.