Fig. 3. sciMETv2 splint ligation optimization and performance.

a Read processing and retention broken down by the three splint designs in the variable splint sciMETv2.SL experiment. For all boxplots, boxes indicate median, 25th and 75th percentiles with min and max lines as 1.5x interquartile range. b UMAP colored by variable splint (left) and composition of clusters by splint and splint by clusters (right). c Cumulative coverage from sampled cells for each method over 100 iterations. Mean values for iterations (left) are shown and include two down sampled variants of the sciMETv2.LA dataset to match the raw read count of the sciMETv2.SL datasets using the H10 (LA, SL-H10 ds.) or N7 (LA, SL-N7 ds.) splints. The distribution of coverage across iterations is shown for the LA, SL-H10 ds. and the SL-H10 datasets (right).