. 2022 Dec 9;12:21314. doi: 10.1038/s41598-022-25747-5

Table 2.

Significant autonomic, metabolomic, and cardiorespiratory fitness variables across age groups.

Variables	Groups
Variables	G_20–29	G_30–39	G_40–49	G_50–59	G_60–69
3-Hydroxyisobutyrate (mM)	0.014 (0.011–0.018)	0.016 (0.014–0.022)	0.015 (0.012–0.020)	0.015 (0.013–0.017)	0.012 (0.009–0.016)^bc
Aspartate (mM)	0.024 (0.018–0.031)	0.025 (0.020–0.032)	0.032 (0.028–0.035)^ab	0.031 (0.029–0.034)	0.031 (0.027–0.038)
Isoleucine (mM)	0.069 (0.060–0.082)	0.077 (0.065–0.084)	0.070 (0.061–0.086)	0.067 (0.059–0.081)	0.056 (0.049–0.063)^abcd
Leucine (mM)	0.098 (0.075–0.112)	0.097 (0.087–0.116)	0.102 (0.084–0.120)	0.096 (0.082–0.112)	0.080 (0.070–0.092)^bc
Valine (mM)	0.232 (0.204–0.274)	0.268 (0.237–0.298)^a	0.249 (0.211–0.292)	0.246 (0.230–0.278)	0.198 (0.189–0.220)^abcd
Hippuric acid (a.u.)	0.486 (0.389–0.692)	0.661 (0.433–0.977)	0.571 (0.394–0.855)	0.676 (0.538–0.771)	1.365 (0.864–2.502)^abcd
10E,12Z-CLA (a.u.)	0.193 (0.129–0.287)	0.615 (0.195–1.587)^a	0.831 (0.252–1.545)^a	0.233 (0.132–0.471)	0.283 (0.139–0.383)^c
VO_2PEAK (mL/kg/min)	32.300 (30.000–37.500)	37.000 (31.550–40.050)	30.950 (27.675–37.950)	29.500 (26.475–33.450)	23.850 (19.975–27.225)^abc
hs-CRP (mg/L)	0.210 (0.070–0.500)	0.760 (0.235–1.505)^a	0.370 (0.160–0.965)	1.190 (0.498–1.808)^a	0.655 (0.228–1.128)^a
TC (mg/dL)	171.000 (139.000–189.000)	179.000 (159.000–197.500)	191.500 (177.000–213.250)^a	203.000 (170.250–207.000)^a	194.000 (171.000–213.000)^a
LDL (mg/dL)	93.000 (65.000–113.000)	102.000 (91.000–121.500)	118.500 (94.000–130.250)^a	115.000 (110.000–132.500)	110.000 (100.500–131.500)^a
Urea (mg/dL)	27.000 (23.000–32.000)	33.000 (29.500–37.000)^a	32.000 (29.000–36.500)	34.000 (30.750–37.750)^a	32.500 (29.250–36.500)^a
HP variance (ms²)	2210.294 (1560.851–3355.204)	1709.864 (929.651–3496.373)	1345.621 (915.377–2210.501)	1189.985 (844.328–1843.296)^a	825.298 (557.824–1243.271)^a
LF (ms²)	811.257 (476.825–1302.738)	677.183 (264.036–1075.832)	460.334 (271.116–983.842)	373.885 (204.137–445.242)^a	176.443 (72.286–385.287)^abc
HF (ms²)	798.676 (429.766–1854.859)	517.024 (326.885–970.092)	340.342 (188.324–589.965)	364.898 (122.537–627.217)	176.384 (93.676–359.366)^ab

Data presented in median and interquartile interval. Logarithm (for hippuric acid, 10E,12Z-CLA, isoleucine, 3-Hydroxyisobutyrate, leucine, HP Variance, LF, and HF), cubic root (for hs-CRP), quadratic (for TC), and inverse (for valine) transformations were used in the statistics. The variables not listed above were used the original data.

10E,12Z-CLA 10E,12Z-octadecadienoic acid, G_20–29 20–29 years old group, G_30–39 30–39 years old group, G_40–49 40–49 years old group, G_50–59 50–59 years old group, G_60–70 60–70 years old group, HF high frequency band, HP heart period, hs-CRP high-sensitivity C-reactive protein, LDL low density lipoprotein, LF low frequency band, TC total cholesterol, VO_2PEAK peak oxygen consumption.

^aDifference with the G_20–29 group.

^bDifference with the G_30–39 group.

^cDifference with the G_40–49 group.

^dDifference with the G_50–59 group.

Tukey’s post hoc at P < 0.01 and effect size with 99% CI for all variables with P < 0.01 in one-way ANOVA.