FIG. 2.

Immunoreactivity of the cytoskeleton-rich (CS) fraction of BEC and binding of B. cepacia. The CS fraction extracted from 10⁴ BEC was separated by SDS-PAGE and Western blotted onto nitrocellulose. Lane 1, molecular mass standards in kilodaltons; lane 2, Coomassie blue staining; lane 3, reactivity with a polyclonal antibody (1:500 dilution) to mixed epidermal cytokeratins; lanes 4 and 5, reactivity with MAbs (0.04 μg of Fab fragments/ml) to CK13 and CK4, respectively; lane 6, overlay with ³⁵S-labeled isolate BC7 (10⁸ CFU/ml) for 1.5 h at 37°C and detection by autoradiography.