FIG. 4.
CS fractions from different cell types. Immunoreactivity with MAb to CK13 and BC7 binding. CS fractions from BEC and other epithelial cells were subjected to SDS-PAGE, and proteins were transferred to nitrocellulose membranes. One blot (A) was incubated with the MAb to CK13 (0.04 μg of Fab fragments/ml), and a separate identical blot (B) was incubated with 35S-BC7 (7 × 108 CFU/ml). CK13 was detected by using a second antibody conjugated to alkaline phosphatase. Bound bacteria were detected by autoradiography. Lane 1, prestained molecular mass standards (in kilodaltons); lanes 2 to 7 represent CS fractions from BEC (0.5 × 104), NHBE (1.1 × 105), A-431 (0.56 × 105), A-549 (5 × 105), NCI-H520 (5 × 105), and HEp-2 (5 × 105) cells, respectively.