FIG. 5.

Binding of B. cepacia to NHBE cells. NHBE cells were grown in 96-well plates until they were 80 to 90% confluent. The wells were blocked with 3% BSA for 1 h at 37°C, washed with PBS, and then incubated with 0.1 ml of B. cepacia (10⁵ to 10⁸ CFU/ml) for 1 h. The wells were washed five times with PBS to remove nonbound bacteria, and the bound bacteria were quantitated by enzyme-linked immunosorbent assay by using an antibody specific for B. cepacia. ●, Cbl-positive isolate BC7; ○, Cbl-negative isolate BC45. The mean ± the standard error of the mean of quadruplicate assays for each bacterial concentration is given.