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Colocalization of bound B. cepacia and CK13 on NHBE cells. NHBE cells were incubated with the mouse MAb to CK13 and FITC-labeled B. cepacia BC7 as described in Materials and Methods, washed, and then incubated with antimouse antibody conjugated with the fluorophore LRSC. Cells were counterstained with Mayer's hematoxylin. Panels a and b represent CK13 localization and B. cepacia binding, respectively. Magnification bar, 10 μm.
