TABLE 2.
Inhibition of BC7 binding to NHBE cellsa
| Inhibitor(s) | Binding (bacteria/10 cells) |
|---|---|
| None (control) | 54 ± 5.1 |
| Mixed epidermal cytokeratins (20 μg/ml) | 3.3 ± 0.53 |
| BEC CS fraction (8.3 μg/ml) | 4 ± 0.43 |
| Nonimmune serum (1:50 dilution) | 50 ± 4.5 |
| Antibody to mixed epidermal cytokeratins (1:50 dilution) | 5.3 ± 0.48 |
| Nonimmune serum absorbed on BEC CS fraction | 53 ± 2.9 |
| Antibody to mixed epidermal cytokeratins absorbed on BEC CS fraction | 16 ± 2.31 |
a
FITC-labeled isolate BC7 (107 CFU/ml) was incubated with NHBE cells grown in Lab-Tek chamber slides for 1 h in the presence or absence of potential inhibitors. The cells were washed and counterstained with hematoxylin, and bacteria were detected by fluorescence microscopy. Bound bacteria per cell were counted for all cells in 10 representative microscopic fields (each field containing at least 25 NHBE cells) and then averaged. Values represent the mean number of bacteria bound ± the standard error of the mean.