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. 2022 Dec 8;45(1):29–43. doi: 10.1007/s11357-022-00705-1

Table 3.

Clinical laboratory tests of the placebo and three NMN-treated groups, comparisons of the three NMN-treated groups to placebo at both baseline and day 60

Placebo, n = 20 300 mg NMN, n = 20 600 mg NMN, n = 20 900 mg NMN, n = 20
Mean ± SD Mean ± SD P* Mean ± SD P* Mean ± SD P*
Hematology
  Hb, g/dL Baseline 12.6 ± 1.8 13.5 ± 1.9 0.12 13.2 ± 2.0 0.26 13.7 ± 1.6 0.04
Day 60 13.0 ± 1.6 13.4 ± 2.3 0.49 14.0 ± 2.2 0.10 13.9 ± 1.9 0.11
  Ht-PCV, % Baseline 38.2 ± 4.8 40.5 ± 4.3 0.12 40.2 ± 5.1 0.20 41.2 ± 4.5 0.05
Day 60 41.3 ± 4.5 41.1 ± 6.0 0.33 43.0 ± 5.6 0.29 42.5 ± 4.8 0.41
  MCHC, g/dL Baseline 32.8 ± 1.2 33.1 ± 1.3 0.37 32.9 ± 1.2 0.83 33.2 ± 0.9 0.17
Day 60 31.8 ± 1.2 32.3 ± 1.2 0.22 32.5 ± 1.2 0.09 32.6 ± 1.0 0.03
  MCV, fL Baseline 83.1 ± 13.0 87.6 ± 8.5 0.21 86.2 ± 6.7 0.36 88.4 ± 9.0 0.15
Day 60 85.0 ± 12.4 91.9 ± 11.6 0.08 88.3 ± 6.8 0.31 88.1 ± 8.6 0.36
  Total leukocyte count-WBC total count, /cm2 Baseline 7160 ± 1538 7325 ± 2132 0.78 7005 ± 1737 0.77 6780 ± 1379 0.42
Day 60 7475 ± 1874 7835 ± 2900 0.64 7930 ± 2489 0.52 7505 ± 1721 0.96
  Absolute neutrophils, /cm2 Baseline 4218 ± 1265 4134 ± 1559 0.85 4068 ± 1245 0.71 3806 ± 1046 0.27
Day 60 4140 ± 1265 4275 ± 1846 0.79 4135 ± 1561 0.99 3910 ± 987 0.53
  Neutrophils, % Baseline 58.5 ± 7.7 55.5 ± 7.7 0.22 57.7 ± 8.2 0.74 55.6 ± 6.9 0.22
Day 60 55.8 ± 7.0 53.3 ± 8.1 0.29 51.7 ± 8.1 0.09 52.3 ± 8.0 0.15
  Lymphocytes, % Baseline 34.1 ± 6.3 37.5 ± 7.4 0.13 34.5 ± 7.3 0.85 36.3 ± 6.2 0.29
Day 60 35.4 ± 7.5 37.1 ± 7.4 0.48 39.2 ± 7.4 0.11 37.3 ± 6.1 0.38
  Monocytes, % Baseline 3.50 ± 1.02 3.80 ± 1.30 0.49 4.10 ± 1.42 0.15 3.80 ± 1.58 0.60
Day 60 5.50 ± 1.49 5.50 ± 1.29 0.94 5.40 ± 1.53 0.93 5.50 ± 1.62 0.88
  Basophils, % Baseline 0.00 ± 0.02 0.00 ± 0.03 0.56 0.00 ± 0.02 1.00 0.00 ± 0.00 0.32
Day 60 0.00 ± 0.02 0.00 ± 0.02 1.00 0.00 ± 0.16 0.40 0.00 ± 0.02 1.00
  Eosinophils, % Baseline 3.80 ± 3.91 3.20 ± 2.29 0.54 3.60 ± 2.89 0.89 4.40 ± 2.57 0.60
Day 60 3.60 ± 2.78 4.20 ± 2.46 0.49 3.60 ± 2.75 0.98 4.80 ± 4.16 0.28
  Platelets, /cm2 Baseline 289,200 ± 61,258 279,450 ± 79,343 0.67 280,450 ± 84,405 0.71 293,450 ± 77,477 0.85
Day 60 303,900 ± 69,230 321,600 ± 86,507 0.48 311,200 ± 85,234 0.77 319,850 ± 87,594 0.53
  PTT, seconds Baseline 11.6 ± 0.6 12.3 ± 1.6 0.07 11.8 ± 0.6 0.30 11.9 ± 0.7 0.07
Day 60 12.0 ± 0.9 12.5 ± 1.4 0.14 11.9 ± 1.0 0.91 11.9 ± 0.7 0.64
  aPTT, seconds Baseline 33.5 ± 3.1 33.0 ± 4.3 0.66 33.1 ± 5.1 0.79 32.6 ± 4.7 0.50
Day 60 34.1 ± 6.6 32.7 ± 3.7 0.44 31.6 ± 3.8 0.16 31.1 ± 3.3 0.08
  RBC, million/cm2 Baseline 4.70 ± 0.94 4.60 ± 0.33 0.72 4.70 ± 0.49 0.90 4.70 ± 0.60 0.99
Day 60 4.90 ± 0.79 4.60 ± 0.69 0.16 4.90 ± 0.54 0.96 4.90 ± 0.66 0.87
Clinical blood, plasma, and serum chemistry
  TG, mg/dL Baseline 156 ± 86 159 ± 70 0.89 157 ± 62 0.96 178 ± 83 0.41
Day 60 161 ± 64 138 ± 72 0.29 135 ± 57 0.19 149 ± 63 0.57
  LDL, mg/dL Baseline 114 ± 30 122 ± 32 0.46 119 ± 22 0.53 132.80 ± 39.70 0.10
Day 60 119 ± 34 116 ± 28 0.82 129 ± 26 0.29 146.00 ± 36.90 0.02
  HDL, mg/dL Baseline 42.4 ± 10.0 43.8 ± 12.3 0.69 41.8 ± 5.6 0.84 40.5 ± 8.2 0.52
Day 60 47.9 ± 14.0 45.1 ± 15.8 0.55 45.9 ± 9.4 0.60 45.2 ± 9.1 0.48
  TC, mg/dL Baseline 171 ± 32 176 ± 32 0.60 179 ± 26 0.37 190 ± 47 0.13
Day 60 180 ± 30 167 ± 28 0.17 185 ± 31 0.66 200 ± 41 0.10
  HOMA-IR Baseline 1.41 ± 0.79 2.28 ± 1.43 0.25 1.70 ± 1.05 0.94 2.01 ± 1.26 0.43
Day 60 2.09 ± 1.40 2.28 ± 1.42 0.13 2.14 ± 1.02 0.89 2.67 ± 1.31 0.62
  Creatine, mg/dL Baseline 0.70 ± 0.19 0.80 ± 0.15 0.67 0.70 ± 0.15 0.72 0.80 ± 0.20 0.24
Day 60 0.80 ± 0.20 0.80 ± 0.19 0.93 0.80 ± 0.20 0.72 0.90 ± 0.21 0.39
  Urea, mg/dL Baseline 17.6 ± 5.3 18.3 ± 5.4 0.91 18.7 ± 5.9 0.57 19.9 ± 7.1 0.25
Day 60 18.7 ± 5.0 18.9 ± 6.1 0.91 18.5 ± 3.2 0.89 21.0 ± 7.1 0.23
  AST, U/L Baseline 23.2 ± 7.0 22.4 ± 6.2 0.71 20.9 ± 5.5 0.26 29.9 ± 21.4 0.19
Day 60 22.4 ± 6.7 21.6 ± 7.0 0.68 22.2 ± 5.4 0.89 22.0 ± 7.2 0.84
  ALT, U/L Baseline 20.6 ± 9.2 23.7 ± 11.7 0.35 17.5 ± 6.7 0.49 32.3 ± 35.8 0.16
Day 60 19.4 ± 7.9 19.6 ± 9.4 0.92 17.7 ± 7.0 0.49 20.2 ± 8.8 0.75
  Alkaline phosphatase, U/L Baseline 84.1 ± 22.4 83.7 ± 22.6 0.95 87.2 ± 22.2 0.67 87.2 ± 22.9 0.68
Day 60 89.8 ± 25.1 96.6 ± 25.9 0.41 90.0 ± 26.5 0.98 94.4 ± 25.9 0.57
  Bilirubin, mg/dL Baseline 0.50 ± 0.24 0.60 ± 0.32 0.56 0.60 ± 0.38 0.42 0.50 ± 0.41 0.70
Day 60 0.60 ± 0.23 0.60 ± 0.26 0.67 0.60 ± 0.27 0.44 0.50 ± 0.43 0.77
  Sodium, mmol/L Baseline 137 ± 4 140 ± 2 0.01 139.8 ± 2.9 0.03 139.20 ± 2.37 0.08
Day 60 140 ± 2 140 ± 2 0.54 140.5 ± 2.2 0.31 140.3 ± 2.7 0.57
  BUN, mg/dL Baseline 8.20 ± 2.46 8.60 ± 8.25 0.67 8.7 ± 2.8 0.56 9.3 ± 3.3 0.25
Day 60 8.80 ± 2.33 8.70 ± 2.91 0.96 8.6 ± 1.5 0.81 9.8 ± 3.3 0.26
  Uric acid nitrogen, mg/dL Baseline 5.40 ± 1.38 5.10 ± 1.21 0.61 4.5 ± 1.7 0.09 5.4 ± 1.2 0.97
Day 60 5.50 ± 1.06 5.10 ± 1.56 0.42 4.4 ± 1.6 0.02 5.2 ± 1.3 0.46
  Chloride, mmol/L Baseline 99.6 ± 4.7 102 ± 3 0.04 102 ± 3 0.10 101 ± 3 0.19
Day 60 102 ± 2 102 ± 3 0.36 102 ± 2 0.93 103 ± 3 0.76
  Potassium, mmol/L Baseline 4.50 ± 0.39 4.10 ± 0.25 0.002 4.30 ± 0.47 0.28 4.40 ± 0.47 0.47
Day 60 4.70 ± 0.32 4.60 ± 0.36 0.49 4.60 ± 0.38 0.33 4.50 ± 0.42 0.10
  Calcium, mg/dL Baseline 9.20 ± 0.44 9.30 ± 0.30 0.45 9.20 ± 0.29 0.83 9.30 ± 0.42 0.45
Day 60 9.40 ± 0.45 9.50 ± 0.40 0.40 9.40 ± 0.37 0.91 9.50 ± 0.49 0.57
  GFR, mL/min Baseline 110 ± 25 105 ± 18 0.41 99.1 ± 15.8 0.09 96.80 ± 17.63 0.05
Day 60 100 ± 24 99.5 ± 22.7 0.90 95.50 ± 18.24 0.47 89.0 ± 16.5 0.08
  Albumin, g/dL Baseline 4.40 ± 0.29 4.40 ± 0.27 0.43 4.30 ± 0.32 0.19 4.40 ± 0.22 0.85
Day 60 4.30 ± 0.32 4.40 ± 0.33 0.47 4.50 ± 0.35 0.17 4.40 ± 0.36 0.48
  Total protein, g/dL Baseline 7.20 ± 0.46 7.10 ± 0.41 0.30 7.20 ± 0.44 0.68 7.20 ± 0.32 0.61
Day 60 7.50 ± 0.48 7.60 ± 0.51 0.57 7.50 ± 0.61 0.45 7.60 ± 0.54 0.76
Urinalysis
  Specific gravity Baseline 1.00 ± 0.01 1.00 ± 0.01 0.33 1.00 ± 0.01 1.00 1.00 ± 0.00 0.03
Day 60 1.00 ± 0.01 1.00 ± 0.00 0.14 1.00 ± 0.01 0.37 1.00 ± 0.01 0.61

aPTT activated partial thromboplastin time; ALT alanine aminotransferase; AST aspartate aminotransferase; BUN blood urea nitrogen; GFR glomerular filtration rate; TC total cholesterol; TG triglycerides; Hb hemoglobin; Ht-PCV hematocrit-packed cell volume; MCHC mean corpuscular hemoglobin concentration; MCV mean corpuscular volume; LDL low-density lipoprotein – cholesterol; HDL high-density lipoprotein – cholesterol; HOMA-IR Homeostatic Model Assessment of Insulin Resistance; RBC red blood cell; PTT prothrombin time

*Statistically significant difference was set at p < 0.05 (paired t test except Mann-Whitney U test for HOMA-IR) vs. placebo