Table 2. .
Parameters characterizing the effect of the components of the tested extracts on calcein leakage in the course of fusion of the vesicles formed from mixtures of DOPC/DOPG/CHOL (40/40/20 mol %) and DOPC/CHOL (80/20 mol %), upon the action of 40 mM CaCl2 and 20 wt % PEG-8000, respectively
| Component, µM | RF, % | II, % | RF, % | II, % | –ΔTm, °C | ΔT1/2, °C |
|---|---|---|---|---|---|---|
| CaCl2 | PEG-8000 | DPPG | ||||
| – | 90 ± 6 | – | 75 ± 5 | – | – | – |
| Quercetin, 20 | 9 ± 1* | 85 ± 2 | 77 ± 11 | –2 ± 5 | 1.2 ± 0.2 | 0.8 ± 0.1 |
| Myricetin, 20 | 31 ± 3* | 58 ± 4 | 76 ± 12 | 3 ± 10 | 1.1 ± 0.2 | 0.6 ± 0.2 |
| Rutin, 20 | 88 ± 1 | –5 ± 2 | 80 ± 3 | –7 ± 6 | 0 | 0 |
| Betulin, 10 | 90 ± 2 | 1 ± 1 | 67 ± 5 | 11 ± 2 | 0.1 ± 0.1a | 0.1 ± 0.1a |
| Lupeol, 10 | 82 ± 9 | 7 ± 8 | 70 ± 4 | 8 ± 2 | 0.1 ± 0.1a | 0.2 ± 0.1a |
a According to [25]; RF is the maximum leakage of the fluorescent marker at the liposome fusion; II is the index of inhibition by major components of the tested extracts of calcium- and PEG-8000-mediated vesicle fusion; ΔTm and ΔT1/2 are changes in temperature and half-width of the peak of the main phase transition of DPPG ((lipid : flavonol and lipid : triterpenoid ratios were 10 : 1 and 50 : 1, respectively); *, p ≤ 0.01 (Mann–Whitney–Wilcoxon criterion, comparison of calcium- or PEG-8000-induced dye leakage from the vesicles in the absence and in the presence of extract components).