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. Author manuscript; available in PMC: 2023 Dec 11.
Published in final edited form as: Cell. 2022 Dec 8;185(25):4770–4787.e20. doi: 10.1016/j.cell.2022.11.014

Figure 7: Mechanism of TRiC directed folding pathway for β-tubulin.

Figure 7:

(A) Model of β-tubulin folding pathway; Nascent chain β-tubulin is captured by Prefoldin in an unfolded state. Red and blue dots on Prefoldin and TRiC show key electrostatic points mediating Prefoldin binding and repositioning into the chamber. See text for additional details. (B) TRiC directed folding pathway of β-tubulin through distinct intermediates. Unfolded domains shown in dotted lines; CCT subunits colored according to their charged state. See text for details. (C) Schematic folding landscape of tubulin: (left) Spontaneous tubulin folding leads to trapped intermediates and/or in aggregates. (right) Prefoldin maintains tubulin unfolded and prevents aggregation. TRiC directs the formation of progressively folded intermediates and prevents off-pathway trapped intermediates.