. Author manuscript; available in PMC: 2023 Dec 11.

Published in final edited form as: Cell. 2022 Dec 8;185(25):4770–4787.e20. doi: 10.1016/j.cell.2022.11.014

Key resources table.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti β-tubulin (Mouse)	ProteinTech	Cat# 66240-1-Ig, RRID: AB 2881629
anti β-tubulin (Rabbit) Polyclonal	Abcam	Cat# AB6046, RRID: AB_2210370
anti β-tubulin (Mouse) Monoclonal C-term AA2 epitope	Millipore Sigma	Cat# 05-661, RRID: AB_309885
anti α-tubulin (Mouse) Monoclonal	Sigma-Aldrich	Cat# T5168, RRID: AB_477579
Custom anti human PFD (Rabbit)	Cocalico Biologicals	N/A
IRDye^® 680RD Goat anti-mouse IgG	LiCOr	Cat# 926-68070, RRID: AB_10956588
IRDye^® 800RD Donkey anti-mouse IgG	LiCOr	Cat# 926-32212, RRID: AB_621847
anti -aTubulin (Mouse)	DSHB Univ. of Iowa	Cat#12G10, RRID: AB 1157911
IRDye^® 680RD Donkey anti-rabbit IgG	LiCOr	Cat# 926-68073, RRID: AB_10954442
IRDye^® 800RD Goat anti-rabbit IgG	LiCOr	Cat# 926-32211, RRID: AB_621843
NativePAGE^™ Novex^® 4–16% Bis-Tris Protein Gels, 1.0 mm, 15 well	Life Technologies	Cat# BN1004BOX
NativePAGE^™ Novex^® 4–16% Bis-Tris Protein Gels, 1.0 mm, 10 well	Life Technologies	Cat# BN1002BOX
Bacterial and virus strains
Rosetta^™(DE3)pLysS Competent Cells	EMD Millipore (Novagen)	Cat# 71403
Max Efficiency DH10Bac Cells	Thermo Fisher Scientific	Cat# 10361-012
Biological samples
N/A	N/A	N/A
Chemicals, peptides, and recombinant proteins
hTRiC	This study	N/A
hTRiC C1GFP	This study	N/A
hTRiC:β-tubulin	This study	N/A
hPFD:β-tubulin	This study	N/A
hPFD:α-tubulin	This study	N/A
TBCA	This study	N/A
TBCB	This study	N/A
Benzonase nuclease	Sigma-Aldrich	Cat# E1014-25KU
DSS crosslinker H12/D12	Creative Molecules Inc.	Cat# 001S
PMSF	Sigma-Aldrich	Cat# P7626-5G
CNBr-Activated Sepharose 4 Fast Flow	GE Healthcare	Cat# 17098101
Apyrase ATPase	Sigma-Aldrich	Cat# A6410-500UN
GTP	Thermo Fisher scientific	Cat# R0461
ATP (magnesium salt)	Sigma-Aldrich	Cat# A9187-1G
Proteinase K	Sigma-Aldrich	Cat# P2308-10MG
Sequencing grade Trypsin	Promega	Cat# V5117
Critical commercial assays
N/A	N/A	N/A
Deposited data
Apo-TRiC map	This study	EMD-32822
Prefoldin:β-tubulin:TRiC complex map	This study	EMD-32823
Prefoldin:β-tubulin:TRiC complex model	This study	7WU7
TRiC:β-tubulin intermediate I model	This study	7TRG
TRiC:β-tubulin intermediate II model	This study	7TTN
TRiC:β-tubulin intermediate III model	This study	7TTT
TRiC:β-tubulin intermediate IV model	This study	7TUB
TRiC:β-tubulin intermediate I map	This study	EMD-26089
TRiC:β-tubulin intermediate II map	This study	EMD-26120
TRiC:β-tubulin intermediate III map	This study	EMD-26123
TRiC:β-tubulin intermediate IV map	This study	EMD-26131
Cross-linking mass spectrometry proteomics data	This study	PXD030590
Human α-tubulin	Adib, R. et al, 2019³⁶	6I2I
Human β-tubulin	Adib, R. et al, 2019³⁶	6I2I
Human γ-tubulin	Aldaz. et al, 2005⁶¹	1Z5V
Tubulin homologue: FtsZ from M. jannaschii	Lowe, et al, 1998⁴⁸	1FSZ
Experimental models: Cell lines
High Five^™ Cells (BTI-TN-5B1-4)	Thermo Fisher scientific	Cat# B85502
Sf9 cells	Thermo Fisher scientific	Cat# 11496015
Experimental models: Organisms/strains
N/A	N/A	N/A
Oligonucleotides
5’ pFB-β-tubulin TTT TAG CGG CCG CCA AAA CAT GAG GGA AAT CGT GCA CAT CC	This study	N/A
3’ pFB-β-tubulin ATT TTT CTA GAT TAT CAG GCC TCC TCT TCG GCC TCC	This study	N/A
5’ pFB-α-tubulin TTT TAG CGG CCG CCA AAA CAT GCG TGA GTG CAT CTC CAT CC	This study	N/A
5’ pFB-α-tubulin ATT TTT CTA GAT TAT CAG TAT TCC TCT CCT TCT TCC TCA CC	This study	N/A
5’ TBCA pST39 AGT TTC TAG ATT TGT TTA ACT TTA AGA AGG AGA TAT ACA TAT GGC CGA TCC TCG CGT GAG	This study	N/A
3’ TBCA pST39 AAT TAG GAT CCT CAT TAG TGA TGG TGA TGA TGG TGG GCT TCT AAC TTC ACT G	This study	N/A
5’ TBCB pST39 AGT TTC TAG ATT TGT TTA ACT TTA AGA AGG AGA TAT ACA TAT GGA GGT GAC GGG GGT GTC	This study	N/A
3’ TBCB pST39 AAT TAG GAT CCT CAT TAG TGA TGG TGA TGA TGG TGA CTG CCT ATC TCG TCC AAC CCG TAG TCC	This study	N/A
5’ TBCB pFB TAA TTC TCG AGC TAT AAA TAT GGA GGT GAC GGG GGT GTC G	This study	N/A
3’ TBCB pFB TAA TTG GTA CCT CAT TAG TGA TGG TGA TGA TGG TGA CTG CC	This study	N/A
Recombinant DNA
pFB-α-tubulin	This study	N/A
pFB-β-tubulin	This study	N/A
pFB-TRiC	Knowlton et al, 2021⁵⁸	N/A
pFB-TBCB	This study	N/A
pST39-TBCA	This study	N/A
pTS-4119 α-tubulin template	Tim Stearns lab	N/A
pTS-3067 β-tubulin template	Tim Stearns lab	N/A
pFB-PFD1,2	Gestaut et al, 2019¹¹	N/A
pFB-PFD3,5	Gestaut et al, 2019¹¹	N/A
pFB-PFD4,6	Gestaut et al, 2019¹¹	N/A
pFB-CCT1-GFP,CCT8	Gestaut et al, 2019¹¹	N/A
pFB-CCT2, CCT4	Gestaut et al, 2019¹¹	N/A
pFB-CCT3, CCT6	Gestaut et al, 2019¹¹	N/A
pFB-CCT5, CCT7-6XHis	Gestaut et al, 2019¹¹	N/A
Software and algorithms
RELION v3.1	Zivanov, J., 2018⁶⁷	http://www2.mrc-lmb.cam.ac.uk/relion
PHENIX	Afonine et al., 2018⁷³	https://www.phenix-online.org
ChimeraX	Pettersen et al., 2021⁷⁴	https://www.cgl.ucsf.edu/chimera
ProMod	Pintilie et al., 2020³⁵	http://cryoem.bcm.edu/cryoem/downloads/segger
Igor Pro 6.22A	Wavemetrics Inc.	https://www.wavemetrics.com
Imagequant V 5.2 and 8.1	GE Healthcare Lifecsciences	https://www.gelifesciences.com
Pymol 1.8.6.2	Schrödinger	https://pymol.org
CryoSPARC v3.2	Punjani et al., 2017⁶⁸	https://cryosparc.com/
Namdinator	Kidmose et al., 2019⁷²	https://namdinator.au.dk/
T-coffee	Di Tommaso et al., 2011⁷⁶	https://tcoffee.crg.eu/
Mega X	Hall, 2013⁷⁷	https://www.megasoftware.net/
Other
Quantifoil R1.2/1.3 200-mesh holey carbon-coated grids	Quantifoil	https://www.emsdiasum.com/microscopy/products/grids/quantifoil.aspx