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| Criteria | |
| Amsel’s diagnostic characteristics (at least three out of four to be present) | Uniform vulval fluid |
| Vulval pH more than 4.5 (on litmus paper test) | |
| Positive Whiff test | |
| Clue cells seen (more than 20% of cells) | |
| Gram-stained vulval smear (Hay/Ison) – Presence of more Molucus or Gardnerella morphotypes with little or no lactobacilli | |
