Fig. 3.

A schematic design of assaying neural activity-dependent functional noncoding SNPs in a co-culture system. The co-cultured excitatory and inhibitory neurons can be stimulated by KCl, followed by scATAC-seq/scRNA-seq to separate Ex and Inh neurons, astrocytes (Ast), and residual NPCs (middle). Cell type-specific regulatory SNPs that affect gene expression (reQTL) and chromatin (ASoC) are identified (right).