TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1 | Prototroph | |
| PAD02 | PAO1 ΔpchD::Tc | This study |
| PAD06 | PAO1 ΔpvdA::ΩSm/Sp | This study |
| PAD07 | PAO1 ΔpchD::Tc ΔpvdA::ΩSm/Sp | This study |
| E. coli | ||
| DH5α | recA1 endA1 gylA96 thi-1 hsdR17 supE44 Δ(lac)U169 (φ80dlac ΔM15) | TOYOBO |
| S17-1 | pro thi recA hsdR Tpr Smr; chromosomally integrated RP4-2-Tc::Mu-Km::Tn7; mobilizer of plasmids carrying the R68-derived Mob region | 35 |
| Plasmids | ||
| pMT5059 | Apr; pBR322 derivative carrying multiple cloning sites and a NotI site | 38 |
| pMT5071 | Kmr Cmr; pMOB3 derivative carrying NotI-flanked Mob cassette with ΩCm gene cartridge | 37 |
| pMT5056 | Apr Tcr; pBend2 derivative carrying Tc gene cartridge flanked with multiple restriction enzyme sites | 38 |
| pHRP316 | Apr Smr/Spr; pSL301 derivative carrying ΩSm/Sp gene cartridge flanked with superpolylinkers | 27 |
| pHT004 | Apr; pMT5059 carrying pchD in EcoRI-BamHI site | This study |
| pHT005 | Apr Tcr; pMT5059::ΔpchD::Tc | This study |
| pHT001 | Apr; pMT5059 carrying pvdA in EcoRI-BamHI site | This study |
| pHT010 | Apr Smr/Spr; pMT5059::ΔpvdA::ΩSm/Sp | This study |
a
Tpr, trimethoprim resistant; Smr, streptomycin resistant; Mob, plasmid mobilization; Apr, ampicillin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant; Tcr, tetracycline resistant; Spr, spectinomycin resistant.