Figure 3.
Pathogenicity analysis of BrYV-HY in N. benthamiana and A. thaliana plants. (A) Schematic representation of the construction of the full-length cDNA clone of BrYV-HY. Two overlapping segments corresponding to the complete genome of BrYV-HY were cloned into the binary expression vector pCass4-Rz between the StuI/BamHI sites by homologous recombination. (B) Symptoms of N. benthamiana and A. thaliana plants inoculated with BrYV-HY. Symptoms were photographed at 4 and 14 days post-inoculation (dpi). Experiments were repeated three times, and 10−15 plants were used for each inoculation.
