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. 2022 Dec 5;11(23):3380. doi: 10.3390/plants11233380

Figure 4.

Figure 4

Detection results of BrYV in inoculated N. benthamiana and A. thaliana at 14 dpi. (A) RT-PCR for the detection of infection by BrYV-HY in the upper leaves of N. benthamiana (1–5) and A. thaliana (6–10) at 2 weeks post-inoculation with the primers BrYV-CP-F/BrYV-CP-R. PC, positive control; NC, negative control. (B) Northern blot analysis of BrYV accumulation in inoculated N. benthamiana and A. thaliana at 14 dpi using a DIG-labeled specific probe targeting BrYV CP coding sequences. Five independent plants were used to extract the total RNAs. The rRNAs below demonstrate the equal loading of the total RNAs.