Figure 1.

WT1 over-expression promotes HL-60 cell resistance to H₂O₂. (A) WT1-overexpressed HL-60 cells (HL-60O cells) were established by Lentivirus transfection, and WT1 expression was detected by Western blot (n = 3). The equivalent cells were incubated for 48 h. (B) Cell viability was detected by MTT assay (n = 6). (C) Relative cell fold was detected by MTT assay. Cells were treated with 100 μM H₂O₂ for 24 h. (D) DNA injury was indicated by the percentage of tail DNA in total DNA in Comet assay (n = 3). (E) Cell apoptosis was tested by Annexin V-FITC/PI dual staining in flow cytometry (n = 3). The data were presented as the mean ± SD. ** p < 0.01 compared to the parent HL-60 cells.