Figure 3.

Shikonin is screened as a WT1 inhibitor. (A) The chemical structure of shikonin and its binding affinities to WT1 in molecular docking. (B) The binding of shikonin to WT1 in DARTS experiment. (C) The effect of shikonin on WT1 protein degradation was detected by the protein half-life test. (D) Shikonin suppressed WT1 protein expression indicated by Western blot in HL-60 cells (n = 3). Cells were incubated with shikonin for 48 h. The data were presented as the mean ± SD. * p < 0.05; ** p < 0.01 compared to the untreated control.