TABLE 2.
L. donovani infection results in a differential increase in the proliferative status of progenitor cells in BALB/c and scid mice
| Cells | Percentage of cells in S phasea
|
|||||
|---|---|---|---|---|---|---|
| BALB/c
|
scid
|
|||||
| CFU-GEMM | CFU-GM | BFU-E | CFU-GEMM | CFU-GM | BFU-E | |
| Spleen | ||||||
| Naïve | 2.6 ± 2.1 | 3.7 ± 1.5 | 4.2 ± 1.6 | 8.0 ± 2.5 | 6.3 ± 1.4 | 6.7 ± 2.6 |
| d42 | 21.7 ± 13.3b | 53.0 ± 3.6 | 36.0 ± 3.6 | 13.7 ± 3.5 | 12.8 ± 2.9 | 11.4 ± 4.1 |
| BM | ||||||
| Naïve | 37.5 ± 2.6 | 42.1 ± 5.2 | 15.7 ± 1.8 | 26.3 ± 3.1 | 30.4 ± 3.2 | 16.9 ± 4.2 |
| d42 | 56.7 ± 2.3 | 65.0 ± 6.2 | 22.3 ± 2.0 | 37.0 ± 4.6 | 42.6 ± 0.9 | 26.3 ± 2.7 |
a
The percentage of spleen and bone marrow (BM) progenitor cells in S phase was determined by a [3H]thymidine kill assay, as described in Materials and Methods. The data are expressed as the percentage of decrease in colony formation following [3H]thymidine treatment compared to the mean number of colonies in control untreated samples and represent the mean ± standard error of the mean of triplicate cultures. d42, 42 days postinfection.
b
Data in bold face indicate statistically significant differences between naïve and infected mice (P < 0.05).