TABLE 3.
L. donovani infection of BALB/c and scid mice induces the accumulation of mRNA for hematopoietic growth factors in the bone marrow
| Cytokine or chemokine | Fold increase in mRNA accumulation over age-matched naïve control (p.i.)
|
|||||
|---|---|---|---|---|---|---|
| BALB/c
|
scid
|
|||||
| 5 h | 14 days | 42 daysb | 5 h | 14 days | 42 daysb | |
| Expt 1 | ||||||
| G-CSF | 1.04 ± 0.14c | 1.20 ± 0.37 | 1.65 ± 0.21d | 1.32 ± 0.21 | 1.10 ± 0.28 | 1.22 ± 0.21 |
| GM-CSF | 1.77 ± 0.11 | 1.30 ± 0.20 | 2.99 ± 0.18 | 1.67 ± 0.29 | 3.99 ± 1.38 | 1.69 ± 0.10 |
| IL-1β | 1.17 ± 0.49 | 0.95 ± 0.57 | 1.32 ± 0.29 | 1.56 ± 0.60 | 1.05 ± 0.18 | 1.09 ± 0.27 |
| IL-3 | 1.00 ± 0.02 | 1.01 ± 0.03 | 0.96 ± 0.41 | 0.67 ± 1.15 | 1.00 ± 0.06 | 1.23 ± 0.41 |
| M-CSF | 0.99 ± 0.16 | 1.45 ± 0.04 | 1.74 ± 0.21 | 1.38 ± 0.01 | 1.04 ± 0.29 | 1.42 ± 0.19 |
| SCF | 1.13 ± 0.22 | 0.98 ± 0.24 | 1.33 ± 0.62 | 1.76 ± 0.70 | 1.04 ± 0.31 | 1.77 ± 0.63 |
| MIP-1α | 1.83 ± 0.09 | 0.80 ± 0.30 | 1.23 ± 0.14 | 2.46 ± 0.70 | 0.85 ± 0.10 | 1.12 ± 0.44 |
| MCP-1 | 1.07 ± 1.59 | 0.96 ± 0.29 | 1.44 ± 0.30 | 0.91 ± 0.13 | 0.73 ± 0.40 | 1.33 ± 0.18 |
| γIP-10 | NDe | ND | ND | ND | ND | ND |
| Expt 2 | ||||||
| G-CSF | 1.23 ± 0.16 | 1.26 ± 0.31 | 1.76 ± 0.22 | 0.97 ± 0.22 | 1.08 ± 0.61 | 1.41 ± 0.12 |
| GM-CSF | 1.87 ± 0.26 | 1.54 ± 0.08 | 1.82 ± 0.3 | 1.48 ± 0.08 | 1.07 ± 0.28 | 1.62 ± 0.34 |
| M-CSF | 1.58 ± 0.46 | 1.79 ± 0.70 | 3.46 ± 0.48 | 0.79 ± 0.30 | 1.01 ± 0.70 | 1.32 ± 0.10 |
| MIP-1α | 1.31 ± 0.10 | 1.16 ± 0.12 | 1.00 ± 0.35 | 1.43 ± 0.15 | 1.66 ± 1.08 | 0.62 ± 0.29 |
mRNA for hematopoietic growth factors was measured by RT-PCR in bone marrow of BALB/c and scid mice infected with L. donovani. After normalizing against expression of HPRT, the fold increase in expression of each cytokine or chemokine was calculated for individual infected mice relative to the mean expression in age-matched naïve controls. In experiment 2, there were no significant changes in expression of IL-1β, IL-3, SCF, MCP-1, and γIP-10. These data are omitted for clarity.
Twenty-eight days for experiment 2.
Values represent the mean ± standard error of the mean for three animals analyzed individually at each time point.
Data in boldface represent significant statistical differences between naïve and infected mice (P < 0.05).
ND, undetectable levels of expression.